March 6, 2024
By Jenna Mertz

A WRI-funded study is determining the health risk of pathogens found in private wells in southwestern Wisconsin. The research builds upon the findings of the Southwest Wisconsin Groundwater and Geology (SWIGG) project, which found livestock manure and human wastewater in private well water from Grant, Iowa and Lafayette counties in 2018 and 2019.

The new research will also shed light on the relationship between groundwater, human and livestock waste, and antibiotic resistance.

Maureen Muldoon in a red jacket with mountains and a glacier in the background
Maureen Muldoon, submitted photo.

Maureen Muldoon, a hydrogeologist with the Wisconsin Geological and Natural History Survey, is leading the study. Having previously worked on the SWIGG study, she’s now investigating how local geology and well construction influence the contamination of private wells.

She says wells in the southwestern part of Wisconsin are particularly vulnerable to contamination due to fractured bedrock, which has a lot of holes and cracks.

“When you drive down Highway 151 towards Iowa and you look on the sides of the road and it just looks like Swiss cheese, kind of yellowy-brown Swiss cheese, that is [fractured bedrock],” said Muldoon. “You can imagine how quickly stuff moves through that.”

This porous, Swiss cheese-like bedrock means wastewater from septic systems and agriculture can quickly enter the aquifer, carrying pathogens that can end up in private wells. The SWIGG study found viral, bacterial and protozoan pathogens in 66 of 138 private wells sampled, including norovirus, salmonella, and multiple species of Cryptosporidium.

Just how many people could get sick from these pathogens is what Tucker Burch, a research engineer with the U.S. Department of Agriculture, is trying to figure out. Using archived water samples collected during SWIGG, Burch can estimate the risk of gastrointestinal illness using a tool called quantitative microbial risk assessment, or QMRA. He likened it to a weather report.

“It’s a forecasting method; it’s a modeling approach,” said Burch. “We’re using the data we have to make an estimate about what’s going to happen.”

A headshot of Tucker Burch
Tucker Burch, submitted photo.

In addition to determining the public’s risk of gastrointestinal illness, Burch and the research team will identify whether pathogens came from human or livestock waste in the upper or lower parts of the aquifer.

Muldoon said well location matters when it comes to water quality. The lower aquifer is generally more protected from contaminants than the upper aquifer due to a layer of rock that restricts the downward flow of water. Certain types of well construction, however, allow water to pass through to the lower aquifer.

“It is not good to connect the upper and lower aquifer in this environment because we are exporting water quality problems to the deep, relatively protected aquifer,” said Muldoon. She is currently gathering and analyzing construction reports for the 138 wells used in the study.

In addition to microbes that cause gastrointestinal illness, the research team is also testing the water for antibiotic resistance genes (ARGs) to learn more about where they come from. ARGs are the building blocks of antibiotic resistance.

“Antibiotic resistance, like any other biological trait, is mitigated by genes,” said Burch. “So bacteria have specific genes that give them different mechanisms to fight the antibiotics.”

Joel Stokdyk, a biologist with the U.S. Geological Survey leading the research team’s inquiry into ARGs, emphasizes that the study isn’t delving into the impacts of ARGs on human health or antibiotic resistant infections.

A headshot of Joel Stokdyk standing outside in front of green bushes
Joel Stokdyk, submitted photo.

“A point worth making is that the detection of the antibiotic resistance gene in someone’s drinking water does not mean it’s in a pathogen, does not mean it could make them sick, but it does help us address these other questions.”

One of those questions concerns how many ARGs come from humans versus livestock. Stokdyk said that’s what makes this research novel.

“The research field knows antibiotic resistance genes come from these sources, but we don’t know how much from each. So that’s one of the gaps we’re trying to address,” said Stokdyk.

Although the research team is currently in the data collection and lab phase of the study, they’re already excited about the results. Knowing the amount and source of ARGs could help tailor current manure management strategies and shape future research in livestock production or human medicine.

Said Burch, “There [are] people out there in the real world that take these results and turn around and use them. And that’s always very satisfying. We’re not just running numbers across a computer screen.”

The post Investigating private well water contamination in southwest Wisconsin first appeared on WRI.

Original Article

News Release | WRI

News Release | WRI

https://www.wri.wisc.edu/news/investigating-private-well-water-contamination-in-southwest-wisconsin/

Jenna Mertz

The University of Wisconsin Water Resources Institute recently provided funds for three new projects that will conclude at the end of June 2025:

Risk From Pathogens and Exposure to Antibiotic Resistance Genes in Private Wells in Southwest Wisconsin, led by Maureen Muldoon at the University of Wisconsin-Madison

Here’s things that are true about the southwestern Wisconsin counties of Lafayette, Grant and Iowa: they are predominantly rural, people living there mostly get their drinking water from private wells and the water sources lie under fractured rock, which means septic systems and agricultural practices can more easily contaminate the water supply. This research team has recent findings of viral, bacterial and protozoan pathogens in 66 of the 138 private wells in the area, but the health risk associated with this contamination is unknown. That’s in keeping with the broader lack of knowledge about the health risk associated with private well water. This project has three objectives 1) quantify the health risk associated with 10 pathogens detected in wells 2) evaluate well construction and geologic factors for pathogen contamination and 3) assess antibiotic resistance genes co-occurrence with human and livestock fecal contamination.

An Experimental Investigation on the Leaching of Per- and Polyfluoroalkyl Substances (PFAS) From Contaminated Soil, led by Shangping Xu at the University of Wisconsin-Milwaukee

The majority of people in Wisconsin get their drinking water from groundwater. This project will attempt to build an understanding of how what are known as “forever chemicals,” per- and polyfluoroalkyl substances (PFAS), might move through soil and into groundwater drinking water sources. The research team will collect soil core samples from several Wisconsin location, including samples based on factors like soil type, properties and PFAS contamination history. They will apply collected rainwater to the soil cores at rates simulating natural conditions. The rainwater flow patterns will be monitored, and leachate will be collected to measure its volume and its PFAS concentrations. If different transport behavior of PFAS within soil cores collected from different sites is observed, the comparison of the soil physicochemical properties and hydrological patterns will provide clues to the key factors that control PFAS mobility within the vadose zone (where the land and the aquifer meet). This work may also yield knowledge of “high risk” and currently overlooked PFAS areas.

Long-Term Threat of Geogenic Contaminants to Water Quality and Quantity in the Midwestern Cambrian Ordovician Aquifer System, led by Matt Ginder-Vogel at the University of Wisconsin-Madison

The Cambrian Ordovician Aquifer System underlies most of Wisconsin. It’s a system with naturally occurring contaminants—uranium, radium, arsenic and manganese. This project seeks to understand the sources and temporal trends of these contaminants because their variations complicate municipal water system management. The research team will identify six study sites, obtain well cuttings and/or core materials from the sites, quantify the presence and prevalence of potential contaminants and then construct models of how the contaminants move in the system. This will help water managers build and manage wells in a way that prevents water users from being exposed to contaminants.

Green field with water in the background.
Wisconsin is rich with surface water. Its groundwater assets are also critical to the economy and people’s health. New groundwater research will serve the state.

Two University of Wisconsin-Madison-based projects kicked off last July and are ongoing with completion targeted for next year:

Aligning the Wisconsin Idea on Water: Interpreting Public Perspectives and Values, led by Michael Cardiff

This project is documenting rural perspectives (attitudes, perception and values) related to groundwater issues, and the variability of these perspectives within the state through “Wisconsin’s Waters Survey”—a community-sourced public survey to be delivered to a range of rural communities. Rural land covers most of the state, overlies the majority of groundwater and the range of issues that may be important to the rural public is vast, from quality concerns such as nitrate and microbial contamination, to quantity concerns that include agricultural irrigation needs and impacts of groundwater to springs and streamflows.

Biomanipulation of Groundwater Flooding, led by Steve Loheide

This project is examining the causes of groundwater flooding, which leads to the loss of farmland and permanent inundation of homes. Such flooding can happen when extremely flat, internally or poorly drained landscapes get hit with a quantity of rain that doesn’t otherwise drain away, infiltrate the soil without flooding or dissipate through the atmosphere. The research team is examining Dane and Columbia counties’ flood records from the 1930s to the present to identify flood causes and how such factors may have changed through time.

 

 

 

 

The post Water Research Projects Announced first appeared on WRI.

Original Article

News Release | WRI

News Release | WRI

https://www.wri.wisc.edu/news/water-research-projects-announced/

Moira Harrington